Wolfdog.org forum - View Single Post - Update about Labs and DNA storage EDTA blood CsW's

mijke · 07-10-2009, 11:51

After my last post about storage:
Maybe the most easy and simple start is that all CsW owners send EDTA blood for DNA storage to the same lab.
So every dog owner remains the official owner of the DNA storage of his dog. And everybody here can announce if they have send DNA for storage in this lab.

And maybe then it is possible to make on this site a list of all CsW with a storage of blood in that lab.

When in future there are interesting test (for example PRA or epilepsy) or a lab needs blood for validation of a test for CsW, we can write about this on the forum.
And every individual owner can decide to cooperate or not.

So on this moment it is maybe an idea only to choose a lab with a good price for only storage and good price and conditions for DNA distillation.
http://www.wolfdog.org/forum/showthread.php?t=11309

Now I can tell something more about the possibilities

In the mean time I did have contact with several labs about their possibilities and conditions around storage and test in other labs. I also had contact with some genetic experts to hear their experiences with labs.
As I did tell before every lab has a commercial goal

And besides tests and storage, also every lab will use some of the EDTA blood for research of their own (only anonymous and of course without names of dogs and owners). But most of the labs will not make this public

I did compare info and find a lab in Germany what was very honest in all information and have good conditions for storage and tests in other labs.

General info about this lab:

It is possible to storage EDTA blood and the DNA isolation without obligation for a test in this lab
They need only 1 ml EDTA blood for this
DNA storage for 25 years can be offered at a price of 33 Euro per sample. (Special offer!)
This service includes a double DNA isolation as well as storage of the EDTA-blood sample
The sender/owner of the dog remains owner of the EDTA blood and isolation
The owner receives a owner number
Of the EDTA blood that is send, they make first an DNA isolation
They always run a double DNA isolation that results in two independent DNA samples that can be stored at different places.
Each isolation sample is 100µl and labs need approx. 5µl to run a test.
That means they could perform 20 DNA tests from each stored DNA isolation.
They can send the DNA isolation for a test to each lab we want
The owner can decide which tests he want to make in other labs
Because they also can store the EDTA blood sample additionally (approx. 1 ml). The number of DNA tests to be performed is almost unlimited!

For the CsW this lab has already this tests: Maligne Hyperthermia and of course making a DNA profile

Maybe we can also discuss more about the possibilities in Bratislava

07-10-2009, 11:51	#1
mijke Senior Member Join Date: Sep 2003 Location: Warnsveld Posts: 2,033	Update about Labs and DNA storage EDTA blood CsW's After my last post about storage: Maybe the most easy and simple start is that all CsW owners send EDTA blood for DNA storage to the same lab. So every dog owner remains the official owner of the DNA storage of his dog. And everybody here can announce if they have send DNA for storage in this lab. And maybe then it is possible to make on this site a list of all CsW with a storage of blood in that lab. When in future there are interesting test (for example PRA or epilepsy) or a lab needs blood for validation of a test for CsW, we can write about this on the forum. And every individual owner can decide to cooperate or not. So on this moment it is maybe an idea only to choose a lab with a good price for only storage and good price and conditions for DNA distillation. http://www.wolfdog.org/forum/showthread.php?t=11309 Now I can tell something more about the possibilities In the mean time I did have contact with several labs about their possibilities and conditions around storage and test in other labs. I also had contact with some genetic experts to hear their experiences with labs. As I did tell before every lab has a commercial goal And besides tests and storage, also every lab will use some of the EDTA blood for research of their own (only anonymous and of course without names of dogs and owners). But most of the labs will not make this public I did compare info and find a lab in Germany what was very honest in all information and have good conditions for storage and tests in other labs. General info about this lab: It is possible to storage EDTA blood and the DNA isolation without obligation for a test in this lab They need only 1 ml EDTA blood for this DNA storage for 25 years can be offered at a price of 33 Euro per sample. (Special offer!) This service includes a double DNA isolation as well as storage of the EDTA-blood sample The sender/owner of the dog remains owner of the EDTA blood and isolation The owner receives a owner number Of the EDTA blood that is send, they make first an DNA isolation They always run a double DNA isolation that results in two independent DNA samples that can be stored at different places. Each isolation sample is 100µl and labs need approx. 5µl to run a test. That means they could perform 20 DNA tests from each stored DNA isolation. They can send the DNA isolation for a test to each lab we want The owner can decide which tests he want to make in other labs Because they also can store the EDTA blood sample additionally (approx. 1 ml). The number of DNA tests to be performed is almost unlimited! For the CsW this lab has already this tests: Maligne Hyperthermia and of course making a DNA profile Maybe we can also discuss more about the possibilities in Bratislava __________________ Vriendelijke groeten, Mijke PS: I am not a moderator anymore!!